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Cell-Free Synthesis of Encephalomyocarditis Virus
We developed a system for complete replication of encephalomyocarditis virus (EMCV) in a test tube by using an in vitro translation extract from Krebs-2 cells. Efficient virus synthesis occurred in a narrow range of Mg(2+) and EMCV RNA concentrations. Excess input RNA impaired RNA replication and vi...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology
2003
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC154999/ https://ncbi.nlm.nih.gov/pubmed/12743313 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.77.11.6551-6555.2003 |
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