Failure To Genotype Herpes Simplex Virus by Real-Time PCR Assay and Melting Curve Analysis Due to Sequence Variation within Probe Binding Sites

Real-time PCR with melting curve analysis of PCR products is a rapid procedure for detecting and genotyping herpes simplex virus (HSV). When testing mucocutaneous samples for HSV by a real-time PCR assay targeting the DNA polymerase gene, we found that some PCR products had atypical melting curves t...

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Detalhes bibliográficos
Main Authors: Anderson, Trevor P., Werno, Anja M., Beynon, Kirsten A., Murdoch, David R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC154719/
https://ncbi.nlm.nih.gov/pubmed/12734260
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.5.2135-2137.2003
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