In Situ Accessibility of Saccharomyces cerevisiae 26S rRNA to Cy3-Labeled Oligonucleotide Probes Comprising the D1 and D2 Domains

Fluorescence in situ hybridization (FISH) has proven to be most useful for the identification of microorganisms. However, species-specific oligonucleotide probes often fail to give satisfactory results. Among the causes leading to low hybridization signals is the reduced accessibility of the targete...

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Bibliographic Details
Main Authors: Inácio, João, Behrens, Sebastian, Fuchs, Bernhard M., Fonseca, Álvaro, Spencer-Martins, Isabel, Amann, Rudolf
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2003
Subjects:
Mycology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC154520/
https://ncbi.nlm.nih.gov/pubmed/12732564
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.5.2899-2905.2003
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