An Improved Protocol for Quantification of Freshwater Actinobacteria by Fluorescence In Situ Hybridization

We tested a previously described protocol for fluorescence in situ hybridization of marine bacterioplankton with horseradish peroxidase-labeled rRNA-targeted oligonucleotide probes and catalyzed reporter deposition (CARD-FISH) in plankton samples from different lakes. The fraction of Bacteria detect...

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Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Sekar, Raju, Pernthaler, Annelie, Pernthaler, Jakob, Warnecke, Falk, Posch, Thomas, Amann, Rudolf
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 2003
Sujets:	Methods
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC154517/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12732568/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.5.2928-2935.2003
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