An Improved Protocol for Quantification of Freshwater Actinobacteria by Fluorescence In Situ Hybridization

We tested a previously described protocol for fluorescence in situ hybridization of marine bacterioplankton with horseradish peroxidase-labeled rRNA-targeted oligonucleotide probes and catalyzed reporter deposition (CARD-FISH) in plankton samples from different lakes. The fraction of Bacteria detect...

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Detalhes bibliográficos
Main Authors: Sekar, Raju, Pernthaler, Annelie, Pernthaler, Jakob, Warnecke, Falk, Posch, Thomas, Amann, Rudolf
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC154517/
https://ncbi.nlm.nih.gov/pubmed/12732568
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.5.2928-2935.2003
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