Quantitative Detection of Methanotrophs in Soil by Novel pmoA-Targeted Real-Time PCR Assays

Methane oxidation in soils is mostly accomplished by methanotrophic bacteria. Little is known about the abundance of methanotrophs in soils, since quantification by cultivation and microscopic techniques is cumbersome. Comparison of 16S ribosomal DNA and pmoA (α subunit of the particulate methane mo...

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Vydáno v:	Appl Environ Microbiol
Hlavní autoři:	Kolb, Steffen, Knief, Claudia, Stubner, Stephan, Conrad, Ralf
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 2003
Témata:	Methods
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC154495/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12732507/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.5.2423-2429.2003
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