Quantitative Detection of Methanotrophs in Soil by Novel pmoA-Targeted Real-Time PCR Assays

Methane oxidation in soils is mostly accomplished by methanotrophic bacteria. Little is known about the abundance of methanotrophs in soils, since quantification by cultivation and microscopic techniques is cumbersome. Comparison of 16S ribosomal DNA and pmoA (α subunit of the particulate methane mo...

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Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Kolb, Steffen, Knief, Claudia, Stubner, Stephan, Conrad, Ralf
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 2003
Sujets:	Methods
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC154495/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12732507/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.5.2423-2429.2003
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