Nalaganje...
Volatile buffers can override the “pH memory” of subtilisin catalysis in organic media
The protonation state and activity of enzymes in low-water media are affected by the aqueous pH before drying (“pH memory”). However, both protonation and activity will change if buffer ions can be removed as volatile or organic-extractable weak acids or bases. With NH(4)OOCH buffers, in which both...
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| Main Authors: | , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
The National Academy of Sciences
1999
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC15440/ https://ncbi.nlm.nih.gov/pubmed/9990001 |
| Oznake: |
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