DNase Pretreatment of Master Mix Reagents Improves the Validity of Universal 16S rRNA Gene PCR Results

DNase I pretreatment of 16S rRNA gene PCR reagents was tested. The DNase I requirement for the elimination of false-positive results varied between 0.1 and 70 IU per master mix depending on the applied Taq polymerase. PCR sensitivity was mostly maintained when 0.1 IU of DNase I was used.

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Heininger, Alexandra, Binder, Marlies, Ellinger, Andreas, Botzenhart, Konrad, Unertl, Klaus, Döring, Gerd
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: American Society for Microbiology 2003
Gaiak:
Bacteriology
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC153871/
https://ncbi.nlm.nih.gov/pubmed/12682181
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.4.1763-1765.2003
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