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Isolation of human monocytes by rosetting with antibody coated human erythrocytes and isopycnic gradient centrifugation.

Human erythrocytes O,Rh+ (D) coated with hyperimmune human anti-serum formed rosettes primarily with monocytes in peripheral blood mononuclear cell suspensions. The rosetting cells (EAhu-FRC), separated on a Lymphoprep gradient were found in the pellet and were up to 95% pure monocytes as judged by...

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Autors principals: Zembala, M, Uracz, W, Ruggiero, I, Lemmel, E M
Format: Artigo
Idioma:Inglês
Publicat: 1982
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1536639/
https://ncbi.nlm.nih.gov/pubmed/6982132
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