Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags

Human macrophages are involved in a plethora of pathologic processes ranging from infectious diseases to cancer. Thus they pose a valuable tool to understand the underlying mechanisms of these diseases. We therefore present a straightforward protocol for the isolation of human monocytes from buffy c...

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Detaylı Bibliyografya
Yayımlandı:J Vis Exp
Asıl Yazarlar: Menck, Kerstin, Behme, Daniel, Pantke, Mathias, Reiling, Norbert, Binder, Claudia, Pukrop, Tobias, Klemm, Florian
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: MyJove Corporation 2014
Konular:
Immunology
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4828059/
https://ncbi.nlm.nih.gov/pubmed/25226391
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51554
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