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Generation of conditional mutants in higher eukaryotes by switching between the expression of two genes

A regulatory system for the in-depth study of gene functions in higher eukaryotic cells has been developed. It is based on the tetracycline-controlled transactivators and reverse tTA, which were remodeled to discriminate efficiently between two different promoters. The system permits one to control...

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Détails bibliographiques
Auteurs principaux: Baron, Udo, Schnappinger, Dirk, Helbl, Vera, Gossen, Manfred, Hillen, Wolfgang, Bujard, Hermann
Format: Artigo
Langue:Inglês
Publié: The National Academy of Sciences 1999
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC15342/
https://ncbi.nlm.nih.gov/pubmed/9927685
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