Generation of conditional mutants in higher eukaryotes by switching between the expression of two genes
A regulatory system for the in-depth study of gene functions in higher eukaryotic cells has been developed. It is based on the tetracycline-controlled transactivators and reverse tTA, which were remodeled to discriminate efficiently between two different promoters. The system permits one to control...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1999
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC15342/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9927685/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.3.1013 |
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