Immunofluorescence Technique Using HeLa Cells Expressing Recombinant Nucleoprotein for Detection of Immunoglobulin G Antibodies to Crimean-Congo Hemorrhagic Fever Virus

A HeLa cell line continuously expressing recombinant nucleoprotein (rNP) of the Crimean-Congo hemorrhagic fever virus (CCHFV) was established by transfection with an expression vector containing the cDNA of CCHFV NP (pKS336-CCHFV-NP). These cells were used as antigens for indirect immunofluorescence...

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Bibliografiske detaljer
Main Authors: Saijo, Masayuki, Qing, Tang, Niikura, Masahiro, Maeda, Akihiko, Ikegami, Tetsuro, Sakai, Koji, Prehaud, Christophe, Kurane, Ichiro, Morikawa, Shigeru
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2002
Fag:
Virology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC153404/
https://ncbi.nlm.nih.gov/pubmed/11825944
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.2.372-375.2002
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