Immunofluorescence Technique Using HeLa Cells Expressing Recombinant Nucleoprotein for Detection of Immunoglobulin G Antibodies to Crimean-Congo Hemorrhagic Fever Virus

A HeLa cell line continuously expressing recombinant nucleoprotein (rNP) of the Crimean-Congo hemorrhagic fever virus (CCHFV) was established by transfection with an expression vector containing the cDNA of CCHFV NP (pKS336-CCHFV-NP). These cells were used as antigens for indirect immunofluorescence...

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Detalles Bibliográficos

Publicado en:	J Clin Microbiol
Autores principales:	Saijo, Masayuki, Qing, Tang, Niikura, Masahiro, Maeda, Akihiko, Ikegami, Tetsuro, Sakai, Koji, Prehaud, Christophe, Kurane, Ichiro, Morikawa, Shigeru
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	American Society for Microbiology (ASM) 2002
Materias:	Virology
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC153404/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11825944/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.2.372-375.2002
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