Recombinant Nucleoprotein-Based Enzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin G Antibodies to Crimean-Congo Hemorrhagic Fever Virus

The full-length nucleoprotein of Crimean-Congo hemorrhagic fever virus (CCHFV; 482 amino acid residues) was expressed as a His-tagged recombinant protein (His-CCHFV rNP) in the baculovirus system. The His-CCHFV rNP was efficiently expressed in insect cells and purified by Ni(2+) column chromatograph...

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Detalhes bibliográficos
Main Authors: Saijo, Masayuki, Qing, Tang, Niikura, Masahiro, Maeda, Akihiko, Ikegami, Tetsuro, Prehaud, Christophe, Kurane, Ichiro, Morikawa, Shigeru
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC130674/
https://ncbi.nlm.nih.gov/pubmed/11980926
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.5.1587-1591.2002
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