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Electrochemical and NMR spectroscopic studies of distal pocket mutants of nitrophorin 2: Stability, structure, and dynamics of axial ligand complexes

WT and leucine → valine distal pocket mutants of nitrophorin 2 (NP2) and their NO complexes have been investigated by spectroelectrochemistry. NO complexes of two of the mutants exhibit more positive reduction potential shifts than does the WT protein, thus indicating stabilization of the Fe(II)–NO...

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Main Authors: Shokhireva, Tatjana Kh., Berry, Robert E., Uno, Elizabeth, Balfour, Celia A., Zhang, Hongjun, Walker, F. Ann
Format: Artigo
Jezik:Inglês
Izdano: The National Academy of Sciences 2003
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC152998/
https://ncbi.nlm.nih.gov/pubmed/12642672
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0536641100
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