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High-Efficiency Generation of Antibiotic-Resistant Strains of Streptococcus pneumoniae by PCR and Transformation
We designed a method by which to generate antibiotic-resistant strains of Streptococcus pneumoniae at frequencies 4 orders of magnitude greater than the spontaneous mutation rate. The method is based on the natural ability of this organism to be genetically transformed with PCR products carrying seq...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2003
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC152537/ https://ncbi.nlm.nih.gov/pubmed/12654655 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.47.4.1257-1261.2003 |
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