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Purification and Characterization of a Prolyl Aminopeptidase from Debaryomyces hansenii
A prolyl aminopeptidase (PAP) (EC 3.4.11.5) was isolated from the cell extract of Debaryomyces hansenii CECT12487. The enzyme was purified by selective fractionation with protamine and ammonium sulfate, followed by two chromatography steps, which included gel filtration and anion-exchange chromatogr...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC152443/ https://ncbi.nlm.nih.gov/pubmed/12513999 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.1.227-232.2003 |
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