Purification and Characterization of Maleate Hydratase from Pseudomonas pseudoalcaligenes

Maleate hydratase (malease) from Pseudomonas pseudoalcaligenes has been purified. The purified enzyme (98% pure) catalyzes the stereospecific addition of water to maleate and citraconate (2-methylmaleate), forming d-(+)-malate and d-(+)-citramalate, respectively. 2,3-Dimethylmaleate was also a subst...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	van der Werf, Mariët J., van den Tweel, Will J. J., Hartmans, Sybe
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1993
Assuntos:	Enzymology and Protein Engineering
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182372/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16349034/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.9.2823-2829.1993
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