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Thermostabilization of Bacterial Fructosyl-Amino Acid Oxidase by Directed Evolution
We succeeded in isolating several thermostable mutant fructosyl-amino acid oxidase (FAOX; EC 1.5.3) without reduction of productivity by directed evolution that combined an in vivo mutagenesis and membrane assay screening system. Five amino acid substitutions (T60A, A188G, M244L, N257S, and L261M) o...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2003
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC152437/ https://ncbi.nlm.nih.gov/pubmed/12513988 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.1.139-145.2002 |
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