Gene Replacement in Mycobacteria by Using Incompatible Plasmids

A simple and efficient delivery system was developed for making targeted gene knockouts in Mycobacterium smegmatis. This delivery system relies on the use of a pair of replicating plasmids, which are incompatible. Incompatible plasmids share elements of the same replication machinery and so compete...

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Bibliographic Details
Main Authors: Pashley, Carey A., Parish, Tanya, McAdam, Ruth A., Duncan, Ken, Stoker, Neil G.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2003
Subjects:
Physiology and Biotechnology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC152386/
https://ncbi.nlm.nih.gov/pubmed/12514036
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.1.517-523.2003
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