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Gene Replacement in Mycobacteria by Using Incompatible Plasmids
A simple and efficient delivery system was developed for making targeted gene knockouts in Mycobacterium smegmatis. This delivery system relies on the use of a pair of replicating plasmids, which are incompatible. Incompatible plasmids share elements of the same replication machinery and so compete...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC152386/ https://ncbi.nlm.nih.gov/pubmed/12514036 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.1.517-523.2003 |
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