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Quantitative NMR analysis of the protein G B1 domain in Xenopus laevis egg extracts and intact oocytes

We introduce a eukaryotic cellular system, the Xenopus laevis oocyte, for in-cell NMR analyses of biomolecules at high resolution and delineate the experimental reference conditions for successful implementations of in vivo NMR measurements in this cell type. This approach enables quantitative NMR e...

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Detalhes bibliográficos
Main Authors: Selenko, Philipp, Serber, Zach, Gadea, Bedrick, Ruderman, Joan, Wagner, Gerhard
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1523310/
https://ncbi.nlm.nih.gov/pubmed/16873549
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0604667103
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