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Functional Analysis by Site-Directed Mutagenesis of the NAD(+)-Reducing Hydrogenase from Ralstonia eutropha

The tetrameric cytoplasmic [NiFe] hydrogenase (SH) of Ralstonia eutropha couples the oxidation of hydrogen to the reduction of NAD(+) under aerobic conditions. In the catalytic subunit HoxH, all six conserved motifs surrounding the [NiFe] site are present. Five of these motifs were altered by site-d...

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Udgivet i:J Bacteriol
Principais autores: Burgdorf, Tanja, De Lacey, Antonio L., Friedrich, Bärbel
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2002
Fag:
Enzymes and Proteins
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC151951/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12399498/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.184.22.6280-6288.2002
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