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Functional Analysis by Site-Directed Mutagenesis of the NAD(+)-Reducing Hydrogenase from Ralstonia eutropha

The tetrameric cytoplasmic [NiFe] hydrogenase (SH) of Ralstonia eutropha couples the oxidation of hydrogen to the reduction of NAD(+) under aerobic conditions. In the catalytic subunit HoxH, all six conserved motifs surrounding the [NiFe] site are present. Five of these motifs were altered by site-d...

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Detalhes bibliográficos
Main Authors: Burgdorf, Tanja, De Lacey, Antonio L., Friedrich, Bärbel
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC151951/
https://ncbi.nlm.nih.gov/pubmed/12399498
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.184.22.6280-6288.2002
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