Trapping of megabase-sized DNA molecules during agarose gel electrophoresis
Megabase DNA molecules become trapped in agarose gels during electrophoresis if the electric field exceeds a few volts per cm. Fluorescence microscopy reveals that these molecules invariably arrest in U-shaped conformations. The field-vs.-size dependence for trapping indicates that a critical molecu...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1999
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC15157/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9892654/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.2.453 |
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