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Trapping of megabase-sized DNA molecules during agarose gel electrophoresis

Megabase DNA molecules become trapped in agarose gels during electrophoresis if the electric field exceeds a few volts per cm. Fluorescence microscopy reveals that these molecules invariably arrest in U-shaped conformations. The field-vs.-size dependence for trapping indicates that a critical molecu...

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Bibliografiske detaljer
Main Authors: Gurrieri, Sergio, Smith, Steven B., Bustamante, Carlos
Format: Artigo
Sprog:Inglês
Udgivet: The National Academy of Sciences 1999
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC15157/
https://ncbi.nlm.nih.gov/pubmed/9892654
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