Chemical ligation of folded recombinant proteins: Segmental isotopic labeling of domains for NMR studies

A convenient in vitro chemical ligation strategy has been developed that allows folded recombinant proteins to be joined together. This strategy permits segmental, selective isotopic labeling of the product. The src homology type 3 and 2 domains (SH3 and SH2) of Abelson protein tyrosine kinase, whic...

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Publikašuvnnas:	Proc Natl Acad Sci U S A
Váldodahkkit:	Xu, Rong, Ayers, Brenda, Cowburn, David, Muir, Tom W.
Materiálatiipa:	Artigo
Giella:	Inglês
Almmustuhtton:	National Academy of Sciences 1999
Fáttát:	Biological Sciences
Liŋkkat:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC15146/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9892643/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.2.388
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