Chemical ligation of folded recombinant proteins: Segmental isotopic labeling of domains for NMR studies

A convenient in vitro chemical ligation strategy has been developed that allows folded recombinant proteins to be joined together. This strategy permits segmental, selective isotopic labeling of the product. The src homology type 3 and 2 domains (SH3 and SH2) of Abelson protein tyrosine kinase, whic...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Xu, Rong, Ayers, Brenda, Cowburn, David, Muir, Tom W.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1999
Soggetti:	Biological Sciences
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC15146/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9892643/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.2.388
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