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Mechanisms associated with cGMP binding and activation of cGMP-dependent protein kinase

Using small-angle x-ray scattering, we have observed the cGMP-induced elongation of an active, cGMP-dependent, monomeric deletion mutant of cGMP-dependent protein kinase (Δ(1–52)PKG-Iβ). On saturation with cGMP, the radius of gyration of Δ(1–52)PKG-Iβ increases from 29.4 ± 0.1 Å to 40.1 ± 0.7 Å, and...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Wall, Michael E., Francis, Sharron H., Corbin, Jackie D., Grimes, Kennard, Richie-Jannetta, Robyn, Kotera, Jun, Macdonald, Brian A., Gibson, Rowena R., Trewhella, Jill
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2003
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC151349/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12591946/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.0534892100
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