A High-Throughput Arabidopsis Reverse Genetics System

A collection of Arabidopsis lines with T-DNA insertions in known sites was generated to increase the efficiency of functional genomics. A high-throughput modified thermal asymetric interlaced (TAIL)-PCR protocol was developed and used to amplify DNA fragments flanking the T-DNA left borders from ∼10...

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Autori principali: Sessions, Allen, Burke, Ellen, Presting, Gernot, Aux, George, McElver, John, Patton, David, Dietrich, Bob, Ho, Patrick, Bacwaden, Johana, Ko, Cynthia, Clarke, Joseph D., Cotton, David, Bullis, David, Snell, Jennifer, Miguel, Trini, Hutchison, Don, Kimmerly, Bill, Mitzel, Theresa, Katagiri, Fumiaki, Glazebrook, Jane, Law, Marc, Goff, Stephen A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society of Plant Biologists 2002
Soggetti:
Genomics Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC151197/
https://ncbi.nlm.nih.gov/pubmed/12468722
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1105/tpc.004630
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