High-Level Expression and Purification of a Truncated Merozoite Antigen-2 of Babesia equi in Escherichia coli and Its Potential for Immunodiagnosis

The gene encoding a truncated merozoite antigen-2 (EMA-2t) of Babesia equi was cloned and highly expressed in Escherichia coli as a glutathione S-transferase fusion protein (G-rEMA-2t). Both G-rEMA-2t and rEMA-2t (after the removal of glutathione S-transferase) had good antigenicity. Either Western...

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Main Authors: Huang, Xiaohong, Xuan, Xuenan, Yokoyama, Naoaki, Xu, Longshan, Suzuki, Hiroshi, Sugimoto, Chihiro, Nagasawa, Hideyuki, Fujisaki, Kozo, Igarashi, Ikuo
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2003
Teme:
Clinical Veterinary Microbiology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC150322/
https://ncbi.nlm.nih.gov/pubmed/12624044
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.3.1147-1151.2003
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