High-Level Expression and Purification of a Truncated Merozoite Antigen-2 of Babesia equi in Escherichia coli and Its Potential for Immunodiagnosis

The gene encoding a truncated merozoite antigen-2 (EMA-2t) of Babesia equi was cloned and highly expressed in Escherichia coli as a glutathione S-transferase fusion protein (G-rEMA-2t). Both G-rEMA-2t and rEMA-2t (after the removal of glutathione S-transferase) had good antigenicity. Either Western...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Huang, Xiaohong, Xuan, Xuenan, Yokoyama, Naoaki, Xu, Longshan, Suzuki, Hiroshi, Sugimoto, Chihiro, Nagasawa, Hideyuki, Fujisaki, Kozo, Igarashi, Ikuo
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2003
Soggetti:	Clinical Veterinary Microbiology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC150322/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12624044/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.41.3.1147-1151.2003
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