Construction and Evaluation of Internal Control DNA for PCR Amplification of Chlamydia trachomatis DNA from Urine Samples

An internal control DNA (ICD) with the same primer binding sequences as the target Chlamydia trachomatis DNA was constructed and evaluated in a PCR assay with immunoenzymatic detection. One hundred urine specimens were tested, and 23 were found to contain inhibitors of the PCR, if not subjected to D...

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Veröffentlicht in:	J Clin Microbiol
Hauptverfasser:	Betsou, Fotini, Beaumont, Katy, Sueur, Jean Marie, Orfila, Jeanne
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2003
Schlagworte:	Bacteriology
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC150278/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12624066/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.41.3.1274-1276.2003
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