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Construction and Evaluation of Internal Control DNA for PCR Amplification of Chlamydia trachomatis DNA from Urine Samples

An internal control DNA (ICD) with the same primer binding sequences as the target Chlamydia trachomatis DNA was constructed and evaluated in a PCR assay with immunoenzymatic detection. One hundred urine specimens were tested, and 23 were found to contain inhibitors of the PCR, if not subjected to D...

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Detalhes bibliográficos
Main Authors: Betsou, Fotini, Beaumont, Katy, Sueur, Jean Marie, Orfila, Jeanne
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC150278/
https://ncbi.nlm.nih.gov/pubmed/12624066
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.3.1274-1276.2003
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