A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA
We describe the use of lentiviral vectors expressing small interfering RNAs (siRNAs) to knock down the expression of specific genes in vitro and in vivo. A lentiviral vector capable of generating siRNA specific for GFP after transduction of 293T-GFP cell lines showed no GFP fluorescence. Furthermore...
Salvato in:
| Pubblicato in: | Proc Natl Acad Sci U S A |
|---|---|
| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
2003
|
| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC149921/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12552109/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.0437912100 |
| Tags: |
Nessun Tag, puoi essere il primo ad aggiungerne!!
|