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A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA

We describe the use of lentiviral vectors expressing small interfering RNAs (siRNAs) to knock down the expression of specific genes in vitro and in vivo. A lentiviral vector capable of generating siRNA specific for GFP after transduction of 293T-GFP cell lines showed no GFP fluorescence. Furthermore...

Täydet tiedot

Tallennettuna:
Julkaisussa:Proc Natl Acad Sci U S A
Päätekijät: Tiscornia, Gustavo, Singer, Oded, Ikawa, Masahito, Verma, Inder M.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: National Academy of Sciences 2003
Aiheet:
Biological Sciences
Linkit:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC149921/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12552109/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.0437912100
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