Development of a 5′ Fluorogenic Nuclease-Based Real-Time PCR Assay for Quantitative Detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis

A 5′ nuclease TaqMan PCR was developed for the quantitative detection of the periodontopathic bacteria Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. The relative numbers of bacteria were measured by the comparative threshold cycle method. This simplified method is a way of obtai...

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Detalhes bibliográficos
Main Authors: Yoshida, Akihiro, Suzuki, Nao, Nakano, Yoshio, Oho, Takahiko, Kawada, Miki, Koga, Toshihiko
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Bacteriology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC149717/
https://ncbi.nlm.nih.gov/pubmed/12574302
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.2.863-866.2003
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