Development of a 5′ Fluorogenic Nuclease-Based Real-Time PCR Assay for Quantitative Detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis

A 5′ nuclease TaqMan PCR was developed for the quantitative detection of the periodontopathic bacteria Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. The relative numbers of bacteria were measured by the comparative threshold cycle method. This simplified method is a way of obtai...

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Bibliografische gegevens

Gepubliceerd in:	J Clin Microbiol
Hoofdauteurs:	Yoshida, Akihiro, Suzuki, Nao, Nakano, Yoshio, Oho, Takahiko, Kawada, Miki, Koga, Toshihiko
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	American Society for Microbiology (ASM) 2003
Onderwerpen:	Bacteriology
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC149717/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12574302/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.41.2.863-866.2003
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