Fluorescence In Situ Hybridization-Flow Cytometry-Cell Sorting-Based Method for Separation and Enrichment of Type I and Type II Methanotroph Populations

A fluorescence in situ hybridization-flow cytometry (FISH/FC)-based method was optimized using artificial mixtures of pure cultures of methanotrophic bacteria. Traditional oligonucleotide probes targeting 16S rRNAs of type I (MG84/705 probe) and type II (MA450 probe) methanotrophs were labeled with...

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Autors principals: Kalyuzhnaya, Marina G., Zabinsky, Rebecca, Bowerman, Sarah, Baker, David R., Lidstrom, Mary E., Chistoserdova, Ludmila
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2006
Matèries:
Methods
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1489643/
https://ncbi.nlm.nih.gov/pubmed/16751544
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00161-06
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