Demethylation and expression of methylated plasmid DNA stably transfected into HeLa cells.
In vitro methylation at CG dinucleotides (CpGs) in a transfecting plasmid usually greatly inhibits gene expression in mammalian cells. However, we found that in vitro methylation of all CpGs in episomal or non-episomal plasmids containing the SV40 early promoter/enhancer (SV40 Pr/E) driving expressi...
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| Yayımlandı: | Nucleic Acids Res |
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| Asıl Yazarlar: | , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Oxford University Press
1999
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148799/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10325422/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.11.2332 |
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