Demethylation and expression of methylated plasmid DNA stably transfected into HeLa cells.
In vitro methylation at CG dinucleotides (CpGs) in a transfecting plasmid usually greatly inhibits gene expression in mammalian cells. However, we found that in vitro methylation of all CpGs in episomal or non-episomal plasmids containing the SV40 early promoter/enhancer (SV40 Pr/E) driving expressi...
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| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
1999
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148799/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10325422/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.11.2332 |
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