PI-PfuI and PI-PfuII, intein-coded homing endonucleases from Pyrococcus furiosus. II. Characterization Of the binding and cleavage abilities by site-directed mutagenesis.

PI- Pfu I and PI- Pfu II from Pyrococcus furiosus are homing endonucleases, as shown in the accompanying paper. These two endonucleases are produced by protein splicing from the precursor protein including ribonucleotide reductase (RNR). We show here that both enzymes specifically interact with thei...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Komori, K, Ichiyanagi, K, Morikawa, K, Ishino, Y
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1999
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148691/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10518608/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.21.4175
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