Enzyme-free cloning: a rapid method to clone PCR products independent of vector restriction enzyme sites.
We describe a simple method for the cloning of PCR products without the need for post-amplification enzymatic treatment. Tailed PCR primer sets are used to create complementary staggered overhangs on both insert and vector by a post-PCR denaturation-hybridisation reaction. The single-stranded overha...
Shranjeno v:
| izdano v: | Nucleic Acids Res |
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| Principais autores: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1999
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148636/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10481038/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.19.e26 |
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