Enzyme-free cloning: a rapid method to clone PCR products independent of vector restriction enzyme sites.

We describe a simple method for the cloning of PCR products without the need for post-amplification enzymatic treatment. Tailed PCR primer sets are used to create complementary staggered overhangs on both insert and vector by a post-PCR denaturation-hybridisation reaction. The single-stranded overha...

Descrizione completa

Dettagli Bibliografici

Pubblicato in:	Nucleic Acids Res
Autori principali:	Tillett, D, Neilan, B A
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1999
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148636/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10481038/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.19.e26
Tags:	Aggiungi Tag Nessun Tag, puoi essere il primo ad aggiungerne!!