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Screening for oligonucleotide binding affinity by a convenient fluorescence competition assay.

A competitive homogeneous quenched fluorescence assay system is described for the high throughput screening of DNA conjugates that bind to single-stranded DNA. Fluorescence signal is generated by competitive binding of the sample molecule to a target strand labelled with a quencher probe, which is o...

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Библиографические подробности
Главные авторы: Harrison, J G, Liu, X, Balasubramanian, S
Формат: Artigo
Язык:Inglês
Опубликовано: 1999
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC148575/
https://ncbi.nlm.nih.gov/pubmed/10446257
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