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Screening for oligonucleotide binding affinity by a convenient fluorescence competition assay.
A competitive homogeneous quenched fluorescence assay system is described for the high throughput screening of DNA conjugates that bind to single-stranded DNA. Fluorescence signal is generated by competitive binding of the sample molecule to a target strand labelled with a quencher probe, which is o...
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Главные авторы: | , , |
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Формат: | Artigo |
Язык: | Inglês |
Опубликовано: |
1999
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Предметы: | |
Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC148575/ https://ncbi.nlm.nih.gov/pubmed/10446257 |
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