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Site-directed mutagenesis of motif III in PcrA helicase reveals a role in coupling ATP hydrolysis to strand separation.

Motif III is one of the seven protein motifs that are characteristic of superfamily I helicases. To investigate its role in the helicase mechanism we have introduced a variety of mutations at three of the most conserved amino acid residues (Q254, W259 and R260). Biochemical characterisation of the r...

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Autori principali: Dillingham, M S, Soultanas, P, Wigley, D B
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1999
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC148564/
https://ncbi.nlm.nih.gov/pubmed/10454638
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