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Gap-Junctional Single-Channel Permeability for Fluorescent Tracers in Mammalian Cell Cultures
We have developed a simple dye transfer method that allows quantification of the gap-junction permeability of small cultured cells. Fluorescent dyes (calcein and Lucifer yellow) were perfused into one cell of an isolated cell pair using a patch-type micropipette in the tight-seal whole cell configur...
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| Formato: | Artigo |
| Idioma: | Inglês |
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Biophysical Society
2006
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1483098/ https://ncbi.nlm.nih.gov/pubmed/16632504 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.072306 |
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