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Gap-Junctional Single-Channel Permeability for Fluorescent Tracers in Mammalian Cell Cultures

We have developed a simple dye transfer method that allows quantification of the gap-junction permeability of small cultured cells. Fluorescent dyes (calcein and Lucifer yellow) were perfused into one cell of an isolated cell pair using a patch-type micropipette in the tight-seal whole cell configur...

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Autor principal: Eckert, Reiner
Format: Artigo
Idioma:Inglês
Publicat: Biophysical Society 2006
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1483098/
https://ncbi.nlm.nih.gov/pubmed/16632504
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.072306
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