A re-investigation of the thio effect at the hammerhead cleavage site.
The effect of introducing a phosphorothioate at the hammerhead cleavage site was investigated using a kinetically well-characterized hammerhead. In buffers containing Mg ion, the RP-phosphorothioate isomer cleaved 2000- to 80 000-fold slower than the SPisomer or the unmodified RNA substrate. Additio...
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| Vydáno v: | Nucleic Acids Res |
|---|---|
| Hlavní autoři: | , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
1999
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148203/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9862968/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.2.479 |
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