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Two Nucleoside Uptake Systems in Lactococcus lactis: Competition between Purine Nucleosides and Cytidine Allows for Modulation of Intracellular Nucleotide Pools

A method for measuring internal nucleoside triphosphate pools of lactococci was optimized and validated. This method is based on extraction of (33)P-labeled nucleotides with formic acid and evaluation by two-dimensional chromatography with a phosphate buffer system for the first dimension and with a...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Martinussen, Jan, Wadskov-Hansen, Steen L. L., Hammer, Karin
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2003
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC148060/
https://ncbi.nlm.nih.gov/pubmed/12591866
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.185.5.1503-1508.2003
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